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Canine and Feline Theriogenology. , pp. 225–242. CANINE TECHNIQUES 5 What techniques are available for pregnancy diagnosis and when are they best used? Anatomy The uterine body is the only organ between the colon and the urinary bladder in the caudal abdomen. The uterine horns are tortuous and lie among the small intestines. Pre-procedure considerations Stage at which pregnancy can be diagnosed varies with technique used. There are few reasons to see potentially pregnant dogs outside this window and, in fact, examination may expose them to disease or otherwise potentially harm the pregnancy.

However, a higher percentage of morphologically normal spermatozoa is desirable as poor morphology is often associated with poor motility and, presumably, with a decreased ability to reach the uterine tube and fertilize ova. There is also some reported correlation between abnormalities of head shape and degree of DNA denaturation within the cell. Place one drop of semen at one end of a glass slide and smear it out as for a blood smear. Let it air-dry and stain with a triple stain, leaving it in each of the three solutions (fixative, safranin, and crystal violet) for 5 min.

Skilled operators may be able to identify the fluid-filled epididymis. There should be no free fluid in the scrotum. Post-care and complications Abnormalities identified may be diagnosed by fine-needle aspirate (see Chapter 9), or histopathology after removal of a biopsy specimen (see Chapter 10) or the entire testis. Testicular ultrasound is a more accurate indicator of true testicular volume than is external measurement of testicular size with a pair of calipers. Supplemental reading Gouletsou PG, Galatos AD, Leontides LS.

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